The carpet in the lab of Herb Overkill is the same as the carpet in the Overlook Hotel in The Shining (1980).
The Kevin in this film is different from the Kevin Gru acknowledges in Despicable Me (2010).
Bob has "heterochromia iridum." His eyes are two different colors.
The leading three minions were designed to resemble the three little girls from the Despicable Me movies: Margo, Agnes, and Edith.
Director Pierre CoffinPierre Coffin voiced all 899 minions in this film.
The language known as "Minionese" which is spoken by the minions is a mix between several languages, including English, Spanish, German, French, Italian, Portuguese, Indonesian, Hebrew, Malay, and gibberish.
During Villian Con, Dr. Nefario can be seen showing off his latest invention, the freeze ray gun, to a young Gru and his mother.